Ni+-Affinity Purification of Untagged cAMP Receptor Protein
نویسندگان
چکیده
منابع مشابه
Ni+-affinity purification of untagged cAMP receptor protein.
The cAMP receptor protein (CRP, also known as CAP) functions as a transcriptional activator at more than 100 different E. coli operons (3). CRP is arguably one of the most well-understood transcriptional activators. At simple CRP-dependent promoters, where CRP is the only activator, transcription activation by CRP involves direct contact with RNA polymerase at two distinct sites. These contacts...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02334bm01